Fig. 4. The Losartan effect on PTCs migration. Hsp70 participation. Scratch wound assay. A) WKY or SHR PTCs and B) SHR PTCs shHsp72 Knockdown were grown to confluence, wounded with a P100 pipette tip and subjected to different treatments. Photomicrographs were taken after 0 and 24 h of incubation. WKY PTCs were used as normotensive controls. C) Wound closure quantification. Bars means ± SEM, n=3. ** p≤0.01 vs SHR PTCs (Cc); ## p≤0.01 vs SHR PTCs (L). ≠≠ p≤0.01 vs SHR PTCs (AII). + p≤0.05 vs SHR PTCs (L+AII).